ABSTRACT
Objective: The Chinese herbal formula Huo-Xiang-Zheng-Qi (HXZQ) is effective in preventing and treating coronavirus disease 19 (COVID-19) infection;however, its mechanism remains unclear. This study used network pharmacology and molecular docking techniques to investigate the mechanism of action of HXZQ in preventing and treating COVID-19. Methods: The Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform (TCMSP) was used to search for the active ingredients and targets of the 10 traditional Chinese medicines (TCMs) of HXZQ prescription (HXZQP). GeneCards, Online Mendelian Inheritance in Man (OMIM), Pharmacogenomics Knowledge Base (PharmGKB), Therapeutic Target Database (TTD), and DrugBank databases were used to screen COVID-19-related genes and intersect them with the targets of HXZQP to obtain the drug efficacy targets. Cytoscape 3.8 software was used to construct the drug-active ingredient–target interaction network of HXZQP and perform protein–protein interaction (PPI) network construction and topology analysis. R software was used to perform Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis. Finally, AutoDock Vina was utilized for molecular docking of the active ingredients of TCM and drug target proteins. Results: A total of 151 active ingredients and 250 HXZQP targets were identified. Among these, 136 active ingredients and 67 targets of HXZQP were found to be involved in the prevention and treatment of COVID-19. The core proteins identified in the PPI network were MAPK1, MAPK3, MAPK8, MAPK14, STAT3, and PTGS2. Using GO and KEGG pathway enrichment analysis, HXZQP was found to primarily participate in biological processes such as defense response to a virus, cellular response to biotic stimulus, response to lipopolysaccharide, PI3K-Akt signaling pathway, Th17 cell differentiation, HIF-1 signaling pathway, and other signaling pathways closely related to COVID-19. Molecular docking results reflected that the active ingredients of HXZQP have a reliable affinity toward EGFR, MAPK1, MAPK3, MAPK8, and STAT3 proteins. Conclusion: Our study elucidated the main targets and pathways of HXZQP in the prevention and treatment of COVID-19. The study findings provide a basis for further investigation of the pharmacological effects of HXZQP.
ABSTRACT
During the COVID-19 pandemic, online health platforms and physicians' online knowledge sharing played an important role in public health crisis management and disease prevention. What influences physicians' online knowledge sharing? From the psychological perspective of stimulus-response, this study aims to explore how patients' visit and patients' consultation influence physicians' online knowledge sharing considering the contingent roles of physicians' online expertise and online knowledge sharing experience. Based on 6-month panel data of 45,449 physician-month observations from an online health platform in China, the results indicate that both patients' visit and patients' consultation are positive related to physicians' online knowledge sharing. Online expertise weakens the positive effect of patients' consultation on physicians' online knowledge sharing. Online knowledge sharing experience weakens the positive relationship between visit of patient and physicians' online knowledge sharing, and enhances the positive relationship between patients' consultation and physicians' online knowledge sharing. This study contributes to the literatures about stimulus-response in psychology and knowledge sharing, and provides implications for practice.
ABSTRACT
Successful prospective remembering involves formation of a stimulus (e.g., bottle of medication and/or place where the bottle is kept)-response (e.g., taking a medication) link. We investigated the role of this link in the deactivation of no-longer-relevant prospective memory intentions, as evidenced by commission error risk. Experiment 1a contrasted two hypotheses of intention deactivation (degree of fulfillment and response frequency) by holding constant the degree of intention fulfillment (e.g., participants responded to one of two target words) while manipulating the number of times the intention was performed. Findings supported the response frequency hypothesis. Experiment 1b employed novel lure trials to examine what "stimulus" participants link the prospective memory response to-target words and/or the salient contextual cue-and compared commission errors to Experiment 1a. Findings suggested the salient context alone does not always function as the stimulus. Collectively these findings, in conjunction with those of Experiment 2 (a within-experiment replication) and a combined analysis, suggest that (a) intention deactivation is facilitated by prior responding (formation/strengthening of stimulus-response links), but additional research is needed to establish the robustness of this effect, and (b) when responding frequently to targets, participants are more likely to bind the response to the context alone than to the target or target/context combination, possibly because they learn to rely on context to predict target occurrence. The latter finding was robust and indicates that deactivation of the appropriate stimulus (target and/or context)-response link may be a critical component of reducing commission errors.